Hi,

I am trying to run Transdecoder.Predict on trascirptome assembly. I get this error in multiple of our transcirptome assemblies. When trying to figure it out, I have been able to "narrow" it down to first 1200 sequences out of 49000 in one of our assemblies (see the file attached).

Here is the error:
Error, len(target_sequence)=33 and pwm length = 0 at /home/software/TransDecoder/util/PWM/../../PerlLib/PWM.pm line 337.
PWM::score_plus_minus_pwm(PWM=HASH(0x55dfafb43ce8), "TAACCAGAAAAATTGGAGTTATGCATGGTGTCT", PWM=HASH(0x55dfaf902878)) called at /home/software/TransDecoder/util/PWM/deplete_feature_noise.pl line 116
Error, cmd: /home/software/TransDecoder/util/PWM/deplete_feature_noise.pl --features_plus xaa.transdecoder_dir/start_refinement.+.features --pwm_minus xaa.transdecoder_dir/start_refinement.-.pwm --out_prefix xaa.transdecoder_dir/start_refinement.enhanced died with ret 65280 No such file or directory at /home/software/TransDecoder/util/../PerlLib/Pipeliner.pm line 185.
Pipeliner::run(Pipeliner=HASH(0x564c471be878)) called at /home/software/TransDecoder/util/train_start_PWM.pl line 141
Error, cmd: /home/software/TransDecoder/util/train_start_PWM.pl --transcripts xaa --selected_orfs xaa.transdecoder_dir/longest_orfs.cds.top_24_longest --out_prefix xaa.transdecoder_dir/start_refinement died with ret 512 No such file or directory at /home/software/TransDecoder/PerlLib/Pipeliner.pm line 185.
Pipeliner::run(Pipeliner=HASH(0x5588d653dcb8)) called at /home/software/TransDecoder/TransDecoder.Predict line 379

File:

test_fasta.txt