Similar to the Beginner Segmentation tutorial, this advanced tutorial will guide you through the segmentation of Nuclei, Cell and Cytoplasm objects with the additional difficulty of having to pre-process the images (perform illumination correction) and finding segmentation settings that work well enough across a large number of different images.

The images used come from the Broad Bioimage Benchmark Collection (https://bbbc.broadinstitute.org/bbbc/BBBC022). They are fields of U2OS cells (10 sites imaged in 5 channels), and represent 5 mock treated wells from a single 384 well plate experiment.

The download bundle for this tutorial lives in a public google drive folder: https://drive.google.com/file/d/18YB8RhfFNKZmYFBd2I0mujJNkcxxEuIZ/view?usp=sharing