Dear,

I have been trying to use Freyja for RSVA and RSVB samples. However, I get some unexpected results, although I'm relatively sure that there should be RSV reads in the sample as it's on reference and clinical samples I tested it.

I downloaded :
freyja update --pathogen RSVa freyja update --pathogen RSVb

Next I run demix:
freyja demix sample_ivar_freyja.variants.tsv sample_ivar_freyja.depth --eps 0.01 --depthcutoff 50 --output sample_ivar_freyja_eps1_depth50_RSVA.demix --pathogen RSVa

I get the following error:
No lineage yml for RSVb found. It may need to be developed if not already present on freyja-barcodes.: miniconda3_rd/envs/wastewater_sars_py3_11/lib/python3.11/site-packages/freyja/data/RSVb_lineages.yml'

So it seems it lacks a file and indeed it's not in the folder. Also I cannot seem to find what I did differently compared to your tutorial. Although the downloading of the barcodes is also not mentioned (?) and i got from an error:
Barcode could not be opened for RSVaPlease try running freyja update --pathogenRSVa if you haven't yet done so Barcode not available, please download with freyja update

I also don't find these lineages.yml in they Freyja barcodes.

Please let me know if you need any more information?
Thanks!