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Update README.md
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@@ -63,7 +63,9 @@ StringTie takes as input a binary SAM (BAM) file which must be sorted by
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reference position. This file contains spliced read alignments such as the
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ones produced by TopHat. If you have a text file in SAM format you should
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first convert it to the BAM format using the samtools view command:
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```
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samtools view -S -b input.sam > input.bam
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```
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Any SAM spliced read alignment (a read alignment across at least one junction)
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needs to contain the tag XS to indicate which strand of the RNA that produced
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this read came from. TopHat alignments already include this tag, but if you use

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