I would like to use PR2 to annotate my environmental eukaryotic sequencing data, and I am working with the QIIME2-DADA2 pipeline. I’m a bit confused about which release file(s) would be most suitable for this purpose.

In particular, I would like to train a custom classifier based on my own primer set. Could you kindly advise me on which PR2 file(s) I should use for this, and whether any formatting or preprocessing steps are necessary?

I appreciate your guidance and look forward to your reply.