Note: For detailed documentation, command reference, and advanced usage, see the docs here.

methurator is a Python package to estimate CpG sequencing saturation for DNA methylation sequencing data.

• Extrapolate CpG discovery beyond observed sequencing depth
• Compute theoretical asymptotes
• Optional bootstrap confidence intervals
• Interactive HTML plots
• BioConda and BioContainer support

pip install methurator

conda create -n methurator_env methurator
conda activate methurator_env

docker pull quay.io/biocontainers/methurator

# Run Chao estimator on BAM file
methurator gt-estimator --genome hg19 my_sample.bam --compute_ci

# Generate plots from the results
methurator plot --summary output/methurator_summary.yml