BIOSZEN is an R/Shiny application for biological data analysis and visualization from Excel workbooks.

It supports complete workflows from data loading and filtering to normalization, statistics, significance annotations, and reproducible exports.

• Plot types: Boxplot, Barplot, Violin, Curves, Stacked, Correlation, Heatmap, Correlation Matrix.
• Statistical workflows: normality and significance with multiple comparison modes and p-value corrections.
• Control-based normalization with replicate-aware logic.
• Replicate management with manual and QC-assisted filtering.
• Metadata import/export to restore analysis state.
• Bundle ZIP exports for reproducibility.
• Growth module for extracting uMax, doub_time, lag_time, AUC, and related metrics.

• Improved growth workflow stability and cancellation behavior.
• Expanded tests for growth, replicate selection, and exports.
• Better consistency in filtering and repeated execution behavior.

• R >= 4.1
• Internet access for first-time package installation

1. Install R (and optionally RStudio).
2. Download and unzip the release bundle.
3. Keep App.R and BIOSZEN-vX.Y.Z.tar.gz in the same folder.
4. Run:
   • RStudio: open App.R and click Source
   • Terminal/PowerShell: Rscript App.R
5. Open the app at the local URL shown in the console.

On first launch, BIOSZEN installs dependencies into a local R_libs folder. Keep this folder to avoid reinstalling packages.

Reference input files are available in the app and in:

• inst/app/www/reference_files/

Templates include:

• Ejemplo_platemap_parametros.xlsx
• Ejemplo_curvas.xlsx
• Ejemplo_parametros_agrupados.xlsx
• Ejemplo_input_summary_mean_sd.xlsx

• English manual: inst/app/www/MANUAL_EN.md
• Spanish manual: inst/app/www/MANUAL_ES.md
• PDF manuals:
  • inst/app/www/MANUAL_EN.pdf
  • inst/app/www/MANUAL_ES.pdf

Both manuals cover:

• Input formats (platemap, grouped, summary)
• Full analysis workflow
• Plot-specific controls
• Statistics and significance workflows
• Heatmap and correlation matrix usage
• Growth module
• Metadata and bundle reproducibility
• Troubleshooting

• Rscript not found: use full path to Rscript or add R to PATH.
• Missing packages: check internet access and review logs.
• Port in use: edit launcher port settings.
• Invalid upload: verify required sheet names and column names.

Szenfeld, B. (2026). BIOSZEN (2.0.0). Zenodo. https://doi.org/10.5281/zenodo.18217522

GPL-3.0