This repository contains the raw atomic coordinate sequence (.pdb) for an autonomously engineered PET-hydrolase structurally designed by Sovryn Oceanus (Autonomous R&D Engine).

Unlike conventional lab-generated extreme-temperature enzymes (e.g., FAST-PETase or LCC operating at 50°C - 70°C), this biological candidate is explicitly calibrated for ester hydrolysis of Polyethylene terephthalate (PET) at 5°C in a marine/brackish salinity profile. It is computationally designed for scalable, unheated, passive in-situ oceanic micro-plastic remediation, eliminating the energy requirements of industrial bioreactor heating.

To irrevocably prevent commercial patent monopolization by third parties, the exact, raw 3D atomic coordinates and amino acid sequence of the oceanus_petase_variant_3 champion have been cryptographically hashed and anchored on the Ethereum Base Layer 2 blockchain.

• Blockchain: Base Mainnet (L2)
• TxID: 0x3ec895c0b9ce325944a40f595a3859dd9e3e605841190c63d11dda27120c3f10
• Physical PDB Sequence Data Hash: 0x3575081cdaab8cf4ecd377f486030fb628d8f6012dd457b046188f3a0e2b600d

Any attempt to commercially patent this sequence is legally invalid due to this cryptographic timestamp.

This physical sequence and its derivatives are distributed under the OCEANUS BIO-TECH DUAL LICENSE (v1.1).

1. Academic & Non-Profit: Free to use, synthesize, and deploy for non-commercial environmental cleanup and university research without restriction.
2. Commercial Exploitation: Strictly prohibited without an explicit commercial license. Industrial recycling corps must license this technology.

See the LICENSE.md file for full legal constraints.

• Target Polymer: Polyethylene terephthalate (PET)
• Reaction Class: Ester Hydrolysis
• Cold Saline Stability Score: 0.730
• Catalytic Proxy Score: 0.756

• oceanus_petase_variant_3_champion.pdb - The raw, forcefully extracted 3D molecular blueprint.
• LICENSE.md - The Dual-License framework binding the PDB file to the blockchain.
• README.md - This file.

• Status: In-Silico Validated / Bounded Desk-Review.
• Constraint: controlled_system_only
• This repository provides the computational blueprint only. Sovryn does not endorse or authorize the open-environment release of this organism without strict BSL-authorized wet-lab synthesis, safety trials, and regulatory compliance checks.